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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Blood Cells Mol Dis. 2010 Mar 17;45(1):67–74. doi: 10.1016/j.bcmd.2010.02.016

Figure 2. Phagocytosis of Ig-opsonized sheep-RBC and human-RBC by human-THP-1 macrophages.

Figure 2

(A) Fresh human and sheep RBC were incubated with antiserum at different dilution ratios and detected by fluorescent secondary antibodies, exhibiting a linear opsonization based on mean fluorescence in flow cytometry. (B) Phagocytosis of shRBC by human-derived THP-1 macrophages at different opsonization level observed by DIC microscopy, which showed a saturable type of response (C). Addition of soluble human-CD47 at μM concentrations showed at most a slight inhibition of phagocytosis of sh-RBC by THP-1 macrophages but not enough to reach hRBC phagocytosis levels at 1/900 antiserum dilution ratio.