Adult OHCs show no difference in FZD3 expression in WT and prestin-KO mice. A. FZD3 (anti-FZD3, green) is located in the cytoplasm of both WT and prestin-KO OHCs. No asymmetrical expression is found in adult OHCs. B. Mean fluorescent intensities of FZD3 staining in the cytoplasmic areas of WT- and KO-OHCs outlined in yellow. Average fluorescence intensity in arbitrary units per cell for WT-OHCs is about 12 (12.2, SD: 3.1), for KO-OHCs about 11 (10.78, SD: 2.39). There is no statistical difference between WT-OHCs and KO-OHCs (p=0.11, N=15 for WT and 14 for KO). Missing OHCs are indicated by an asterisk. Bar length: 23.8 μm.