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. Author manuscript; available in PMC: 2011 Apr 30.
Published in final edited form as: J Immunol Methods. 2010 Mar 19;356(1-2):1–5. doi: 10.1016/j.jim.2010.03.005

Figure 4. Binding characteristics of anti-LukS-PV pre-incubated with DEPC to rLukS-PV in YCP media and bacterial culture supernatants.

Figure 4

ELISA was carried out as described in Figure 1. Shown is the binding of 2 μg/ml rLukS-PV in dialyzed YCP media to anti-LukS-PV (closed squares, solid lines) and the binding of 2 μg/ml rLukS-PV in dialyzed SA109 supernatant to DEPC-treated anti-LukS-PV (dashed line, open diamonds). Controls shown are dialyzed SA-109 culture supernatants binding to anti-LukS-PV (solid circles) and dialyzed SA-109 culture supernatants containing 2 μg/ml rLukS-PV binding to anti-LukS-PV (open triangles).