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. 2010 May 31;189(5):871–883. doi: 10.1083/jcb.201001008

Figure 3.

Figure 3.

siRNA depletion of AP-2 decreases internalization of 125I-EGF to a various degree in cells expressing wtEGFR, WTΔAP2, and 21KR receptors. (A) Internalization rate constants (ke) were measured using 1 ng/ml 125I-EGF in wtEGFR, WTΔAP2, and 21KR-expressing cells that were transfected with nontargeting siRNA (NT) or siRNA targeting µ2 subunit of AP-2. Bars represent SEM from four experiments. (B) Lysates of cells used in internalization experiments described in A were probed by Western blotting (WB) with Ab32 to β-adaptin and actin antibody (loading control). All images are from the same Western blot. The efficiency of siRNA depletion (KD%) was calculated as a percentage of the intensity of the β-adaptin signal in depleted cells to that intensity in nontargeting siRNA–transfected cells (both signals were first normalized to the loading control). Black lines indicate that intervening lanes have been spliced out.