Figure 8.
Pom33 is required for normal pore density in the daughter nucleus in telophase. (A, a) Nup84-GFP fluorescence was analyzed by confocal microscopy in the indicated yeast strains. 3D reconstructions of total fluorescence from 13 planes covering the entire nuclei are shown. The cell shape of dividing cells is outlined in white. Bar, 5 µm. (b) The ratios of the total fluorescence intensity of Nup84-GFP in the mother versus the bud nuclei, quantified in telophase cells, are represented as box plots using KaleidaGraph (see Materials and methods). Mean values (m) and SD of the M/B ratios are indicated below the corresponding strains. *, 10−10 < P < 5 × 10−3 compared with wt using the Student’s t test; ***, P < 10−10. The total number of cells quantified is indicated (n; arising from at least two independent experiments). (B) M/B ratio distribution of Nup84-GFP fluorescence in wt and pom33Δ cells at early anaphase, telophase, and G1 stages of mitosis was plotted as in A. Confocal images of Nup84-GFP at the corresponding stages, revealed by the cell shape outlined in white, are shown on top. Bar, 2 µm.