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. 2010 May 31;189(5):795–811. doi: 10.1083/jcb.200910043

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© 2010 Chadrin et al.

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Figure 9. — POM33 deletion impairs Nup84-GFP incorporation into NPCs upon Nup170 depletion. (A) Growth properties of wt, nup157Δ, and pom33Δ cells expressing NUP170 under the control of the P_MET3 repressible promoter in a NUP84-GFP background. Serial dilutions were spotted on selective media lacking methionine (−Met) or on YEPD (+Met). (B) The localization of Nup84-GFP was analyzed in the indicated strains by spinning disk confocal microscopy before (−Met) or after a 6-h repression of NUP170 expression (+Met). Cytoplasmic Nup84-GFP foci are observed upon repression of NUP170 in nup157Δ and to a lower extent in pom33Δ cells (arrows, foci in mother cells; arrowheads, foci in buds). The percentage of cells with Nup84-GFP cytoplasmic foci is indicated. Bar, 5 µm.