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. Author manuscript; available in PMC: 2011 May 7.
Published in final edited form as: Vaccine. 2010 Mar 21;28(21):3706–3713. doi: 10.1016/j.vaccine.2010.03.015

Figure 3.

Figure 3

T cell immune response promoted by chimeric peptide immunization in HLA-A2.1 transgenic mice. NDCP15–18 represented TT helper fused CRPVE1/303–11, PADRE fused CPRVE1/303–311, TT helper fused HPV16E7/82–90 and PADRE fused HPV16E7/82–90 respectively. Two HLA-A2.1 transgenic mice were immunized with each chimeric peptide subcutaneously twice with a two-week interval. One week after booster immunization, spleens were harvested and cultured in vitro with HLA-A2.1 mouse dendritic cells pulsed twice with corresponding peptides. The bulk CTLs were then tested for intracellular interferon gamma secretion. No significant increase in IFNr secreting CD8 T cells was in any of the mice (P>0.05, unpaired student t test) when compared with a reference peptide HIVGagp17/77–85. Therefore, chimeric peptides failed to stimulate specific immune responses in HLA-A2.1 transgenic mice in this study.