Fig. 3.

Genetic crosses by transformation of a recipient strain using genomic DNA from a donor strain (Vlamakis et al., 2004). A genetic cross between two strains is indicated by a cross (×). For each cross (a, b and c), indicated on the left are the strains and the genotypes for both the donor (upper) and the recipient (lower). cheW7 represents the wild-type allele and cheW7* represents the suppressor allele (cheW7-1). 4984 represents MXAN4984. The flag denotes the che7-linked Kan^R marker used for selection in the cross. The lollipop denotes the MXAN4984-linked Kan^R marker used for selection in the cross. A break (//) between two genes indicates a chromosomal distance of ∼275 kb between difA and cheW7 or ∼2 Mb between MXAN4984 and difA. The two possible genotypes from each cross are shown on the right. The number of transformants with each genotype as determined by the phenotypes (EPS⁺ or EPS⁻) is shown on the far right (see Methods).