Table 1.
M. xanthus strains and plasmids used in this study
| Strain or plasmid | Genotype/description | Reference or source |
|---|---|---|
| Strains | ||
| DK1622 | Wild-type | Kaiser (1979) |
| DK10407 | ΔpilA : : TetRdifA+ | Wall et al. (1998) |
| LS308 | Tn5Ω1407 difA+ | Shimkets (1986a) |
| SW504 | ΔdifA-1 | Yang et al. (1998) |
| YZ101 | ΔdifA-1 cheW7-1 | This study |
| YZ601 | ΔdifA-2 | Xu et al. (2005) |
| YZ615 | ΔdifA-1 ΔdifE cheW7-1 | This study |
| YZ617 | ΔdifA-1 ΔdifC cheW7-1 | This study |
| YZ647 | ΔdifA-1cheW7-1 ΔpilA : : TetR | This study |
| YZ648 | ΔdifA-1 ΔpilA : : TetR | This study |
| YZ651 | ΔdifA-1 cheW7-1 MXAN4984 : : pWB513 | This study |
| YZ666 | ΔdifA-2 cheW7-1 | This study |
| YZ684 | ΔdifA-1 cheW7-1 che7 : : pWB521 | This study |
| YZ687 | ΔdifA-2 che7 : : pWB521 | This study |
| YZ688 | ΔdifA-2 che7 : : pWB515 | This study |
| YZ697 | ΔdifA-2 che7 : : pWB530 | This study |
| YZ1600 | ΔdifA-2 che7 : : pWB531 | This study |
| YZ1604 | ΔdifA-2 ΔcheW7 Δmcp7 | This study |
| YZ1605 | ΔdifA-2 ΔcheW7 Δmcp7 attB : : pWB426 | This study |
| YZ1612 | ΔdifA-2 ΔcheW7 Δmcp7 attB : : pWB433 | This study |
| YZ1613 | ΔdifA-1 cheW7-1 Δmcp7 | This study |
| YZ1614 | ΔdifA-2 attB : : pWB433 | This study |
| YZ1615 | ΔdifA-2 ΔcheW7 attB : : pWB435 | This study |
| YZ1617 | ΔdifA-1 cheW7-1 Δmcp7 attB : : pWB433 | This study |
| YZ1618 | difA+cheW7-1 ΔpilA : : TetR | This study |
| YZ1619 | ΔdifA-2 ΔcheW7 | This study |
| YZ1625 | ΔdifA-2 ΔcheW7 attB : : pWB433 | This study |
| BY132 | ΔdifA-1 cheW7-1 cpc7 : : magellan4 | This study |
| Plasmids | ||
| pZErO-2 | Cloning vector, KanR | Invitrogen |
| pMycoMar | magellan4 mutagenesis vector | Rubin et al. (1999) |
| pBJ113 | Cloning vector, KanR, galK | Julien et al. (2000) |
| pBY132 | magellan4 isolated from BY132, KanR | This study |
| pWB116 | ΔdifA-2 in-frame deletion, pBJ113 | Xu et al. (2005) |
| pWB425 | Expression vector, KanR, intP (Mx8 attP) | Unpublished |
| pWB426 | SacI–NotI che7 fragment in pWB425 | This study |
| pWB433 | mcp7 PCR fragment in pWB425 | This study |
| pWB435 | cheW7-1 PCR fragment in pWB425 | This study |
| pWB513 | Fragment ∼2.3 Mb from che7 in pZErO-2 | This study |
| pWB515 | SacI–NotI che7 fragment in pZErO-2 | This study |
| pWB521 | Fragment downstream of che7 in pZErO-2 | This study |
| pWB530 | SacI–PstI che7 fragment in pZErO-2 | This study |
| pWB531 | ClaI–NotI che7 fragment in pZErO-2 | This study |
| pWB534 | ΔcheW7 mcp7 in-frame deletion in pBJ113 | This study |
| pWB552 | ΔcheW7 in-frame deletion in pBJ113 | This study |
| pWB553 | Δmcp7 in-frame deletion in pBJ113 | This study |