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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Dev Biol. 2008 Aug 6;323(1):19–30. doi: 10.1016/j.ydbio.2008.07.034

Figure 4. Sox9-Haploinsufficiency Does Not Result in Impaired Terminal Differentiation of Endocrine Cells.

Figure 4

Immunofluorescence staining for markers of differentiated endocrine cells, including GLUT2, PC1/3, IAPP, MAFA, PDX1, NKX6.1, ISL1 and HB9 reveals all markers to be equally expressed in insulin+ cells of Sox9 heterozygous mutant (Sox9+/Δpan) (E–H; M–P) and wild-type (WT) (A–D; I–L) pancreas at e18.5. qRT-PCRs performed on pooled mRNA from 200 isolated islets per genotype, confirm that islet Glut2 and MafA mRNA levels are similar in two 2-month-old Sox9+/Δpan and WT mice. The values shown represent mean values ± SEM (standard error of the mean) (n=3) (Q). Scale bar = 50µm.