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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Dev Biol. 2008 Aug 6;323(1):19–30. doi: 10.1016/j.ydbio.2008.07.034

Figure 5. Endocrine Progenitors are Two-Fold Reduced in Sox9 Heterozygous Mutant Pancreata.

Figure 5

Numbers of NGN3+ endocrine progenitor cells are 50% reduced in the Sox9 heterozygous mutant (Sox9+/Δpan) compared to wild-type (WT) pancreas (n=3) both as a proportion of total (DAPI+) pancreatic epithelial cells at e12.5 (A–C; pancreatic epithelium highlighted by yellow dashed line in A,B) and per square millimeter of pancreas tissue at e15.5 (D–F). Chromatin immunoprecipitation studies of cross-linked chromatin from dissected pancreata at e15.5 with anti-SOX9 antiserum show that SOX9 occupies sequences in the Neurog3 promoter. Three fragments, as previously described by Lynn et al. 2007, were amplified by PCR from either input DNA, DNA precipitated with IgG or anti-SOX9 antiserum. Sequences from the GABA-A receptor intron 5 were amplified in the control reaction (G). qRT-PCR analysis of DNA precipitated with anti-SOX9 antiserum shows a 3–4-fold enrichment over DNA precipitated with IgG (n=4) (H). The values shown represent mean values ± SEM (standard error of the mean). AMY, amylase. (A,B,D,E) Scale bar = 50µm.