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. Author manuscript; available in PMC: 2010 Nov 27.
Published in final edited form as: Nature. 2010 May 27;465(7297):492–496. doi: 10.1038/nature09075

Figure 1. Reducing CD95 or CD95L expression inhibits cell proliferation of cancer cells.

Figure 1

a-f, Growth of different cell lines infected with the CD95 specific shRNA lentivirus CD95shRNA#6 (R#6). Inserts show the total CD95 expression levels of cells expressing scrambled control (vec) or R#6 as determined by western blot analysis. A similar effect was also found with a CD95 expressing variant of the neuroblastoma cell line NB4 (not shown). Apoptosis sensitivity of HeyA8 vec and R#6 cells by LzCD95 ligand treatment was determined by quantifying DNA fragmentation (a). g, HepG2 cells were stably infected with three different CD95L specific shRNA lentiviruses and CD95L mRNA was quantified using real time PCR. h, Growth of cells in g over 5 days. i, Growth of different cell lines infected with the L#4 virus. Proliferation of cells was examined by SRB assay (a-f and h and i). * p<0.05, ** p<0.01, *** p<0.001. Values in graphs in a to i represent mean -/+ s.d. from three independent experiments.