Table 1.
Cleaved Caspase-3 and PI staining as a function of anti-CD9, HB-EGF and EGF treatment of mouse embryonic stem cells
Anti-CD9 | Anti-CD9 (%) | Anti-CD9 + HB-EGF | HB-EGF + EGF | EGF | Control | |
---|---|---|---|---|---|---|
Cleaved caspase-3 | 80 ± 3.6 | 31 ± 2.1 | 42 ± 1.0 | 6 ± 0.3 | 7 ± 0.5 | 5 ± 0.8 |
PI | 78 ± 4.2 | 31 ± 4.0 | 50 ± 2.2 | 11 ± 1.7 | 15 ± 1.7 | 11 ± 3.4 |
Data are presented as the percentage of cells which are positive for cleaved caspase-3 and PI. Comparisons between groups were determined using Tukey multiple comparison tests, using cell counts from n = 3 independent experiments (p < 0.05).