Table 1. Primers for PCR amplification of zebrafish CRX from genomic DNA.

Forward primer (5′-3′)	Reverse primer (5′-3′)	Target	Temp (anneal) (°C)	Size (bp)
CTCATAGTGTCCCGCTCCTGTCC	GCTCTCTCTCTCCCTCCCTCTCTCAC	exon 1 : 5′	60	364
TTCTCGCATATAGACATTTATTTG	ATAAAACGTCTAACAGAAACACCATTA	exon 1 : 3′	55	471
AACCTCCTGAATTGTTTTAAGCTG	TGTTGAGAAGGATGTGTGAGAGGC	exon 2	55	242
ATCCATCCATTCATCCATCTAACAC	CTCTCTCACTTTCTTTTCTACAGCAC	exon 3 : 5′	55	990
ACGGTCAGCCCTCTTCCTACAGCC	GGAAGGCTCAGATATAGGGGTGG	exon 3 : mid	60	725
GGAAGGAAAGATGCGTGAAGAAG	AACCTTCAGCTCCCCTCTGTACGTG	exon 3 : 3′	60	933
TTCTCGCATATAGACATTTATTTG	TGTCTGGATAGCGTGTTTTGGTGA	intron 1	62	1243
ACCACCTTCACTCGCACCCAG	TGTAGGAAGAGGGCTGACCGTAGC	intron 2	62	1026-1064
AACCTGCCAGAGGGTTACT	TCAACAGATGGCTTCAGTGC	Z13833	50	177 [33]

Oligonucleotide primers used for PCR amplification of zebrafish crx for sequence analysis were designed using Primer3 (Primer3) or Vector NTI (Invitrogen Corp.) software and the published zebrafish crx cDNA sequence (GenBank accession number AF503443) [4] and obtained from IDT as standard, desalted oligonucleotides (25 nmole synthesis). Annealing temperatures were optimized using a gradient program on a MJ Research DNA Engine thermocycler (BioRad).