Skip to main content
. Author manuscript; available in PMC: 2011 May 11.
Published in final edited form as: Circulation. 2010 Apr 26;121(18):1992–2000. doi: 10.1161/CIRCULATIONAHA.109.909093

Figure 6. Cardiac-resident c-kit+ cells from normal adult hearts fail to undergo cardiomyogenic differentiation when transplanted into infarcted adult mouse hearts.

Figure 6

c-kit+ cells from ACT-EGFP/MHC-nLAC adult double transgenic hearts were transplanted into an infarcted non-transgenic recipient heart. The heart was harvested 13 days later, fixed and processed for β-galactosidase activity (blue), EGFP (green) and α-actinin (red) immune reactivity. EGFP-expressing cells were readily detected; however these cells lacked sarcomeric structure when examined by fluorescence microscopy and also lacked β-galactosidase activity when visualized under bright field illumination (inset). Bar = 20 microns in the fluorescent image, 20 microns in the bright field inset. Bright field, single color fluorescence and merged images for these cells are shown in the Supplemental Data section.