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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Mol Cell Cardiol. 2009 Jul 15;48(1):211–219. doi: 10.1016/j.yjmcc.2009.07.003

Figure 1.

Figure 1

Production of inducible heart-specific Clcn3 knock-out mice. (A) Diagrammatic representation of the pFloxP-flp-neo-Clcn3 targeting construct with exon 3 flanked by loxP sites. (B) Southern blot of genomic DNA from non-transfected (wild-type) and transfected (2H1) ES cells digested with PstI. A single 6.0kb was detected in wild-type DNA, while a 6.0 and 3.4kb band was detected in the 2H1 targeted cell line. (C) Resultant mice were genotyped for targeted Clc3n gene. The wild-type Clcn3 allele produced a 467bp band while the targeted allele produced a 746bp band. (D) Loss of the neomycin gene after FLPe recombination was confirmed by PCR. (E) PCR was used genotype mice at the Clcn3 locus and for the aMHC-atTA and TetO-Cre transgenes.

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