Figure 3.
ClC-3 protein expression in heart and brain of cardiac-specific ClC-3 inducible knock-out mice. (A) Western analysis of whole heart and brain from age-matched cardiac-specific ClC-3 inducible knock-out mice, either maintained on doxycycline, or off doxycycline for 3 weeks. Note whole heart was used for the Western, so non-cardiomyocytes cell types are present. (B) isolated atrial and (C) ventricular cardiomyocytes from age-matched cardiac-specific ClC-3 inducible knock-out mice, on doxycycline, or off doxycycline for 3 weeks. In each pair of images the left panel is probed with anti-ClC-3 (green) and the right panel is probed with anti-alpha-actinin (red). Scale bar in B = 5 μm.