Figure 7.
Representative current traces in isotonic condition and under hypotonic challenge in the freshly isolated ventricular myocytes from age-matched cardiac-specific ClC-3 inducible knock-out mice. (A) Mice maintained on doxycycline, and (B) mice after 3 weeks off doxycycline. (C) is a summary of current densities under isotonic and hypotonic solutions, recorded at +80 mV and −80 mV, in freshly isolated atrial myocytes from age-matched cardiac-specific ClC-3 inducible knock-out mice, either maintained on doxycycline (n=13, 4 mice), or off doxycycline for 3 weeks (n=20, 4 mice). Open boxes, under isotonic conditions, filled boxes under hypotonic conditions. Black boxes on doxycycline, and grey boxes off doxycycline for 3 weeks. ** p<0.01, hypotonic-induced VSOAC current densities compared to isotonic conditions. ## p<0.01, hypotonic-induced VSOAC current densities compared between, on doxycycline, and 3 weeks off doxycycline using ANOVA.