Figure 1.

Schematic of the Protein Analysis Process. Cell-free expression of the cPKA fusion protein was confirmed by western blot analysis and binding to a fluorescent HaloTag ligand. Kinase activity was measured after immobilization from cell-free reactions in the presence and absence of cPKA inhibitors. The “bead” in the figure represents the HaloLink magnetic resin and the “HT” refers to the HaloTag protein at the amino-terminus of the cPKA protein.