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. 2010 May 19;5:25–36. doi: 10.4137/aci.s4732

Figure 6.

Figure 6

Inhibition of HaloTag-cPKA expressed in Insect Cell Extract. HaloTag-cPKA protein was expressed from the pF25 N HT plasmid in the TNT T7 Insect Cell Extract. After immobilization, 5 μl of particle suspension were added per well of the 96 well plate. Reactions containing positive control human rPKA (0.001 units, 2.3 ng) were also performed. The final concentration of all compounds was 10 μM. Control reactions contain only kinase buffer (KB) or DMSO (1% final concentration). All reactions were done in triplicate. The average fluorescent signal is plotted and the error bars represent ± 1 S.D.