Table 2.
KRAS Mutations Reported by Mutation Analysis Method
| Sample | Direct Sequencing (Amgen) | Allele-Specific PCR (HistoGeneX/DxS) | Direct Sequencing (Gentris) | Allele-Specific Hybridization (Invitek) | Allele-Specific PCR Extensions (Genzyme) | Picotiter Plate Sequencing (Agencourt)* |
|---|---|---|---|---|---|---|
| Samples for which a KRAS sequence determination was made, n | 40 | 40 | 32 | 27 | 35 | 32 |
| Total WT, n | 20 | 21 | 18 | 3 | 15 | 15 |
| 1 | G13D | G13D | G13D | G13D | G13D | G13D (4219/7836; 0.54)** |
| 2 | WT | WT | WT | Inconclusive† | WT | WT (8880)** |
| 3 | G13D | G13D | 12WT, no call 13† | G13D | G13D | G13D (1679/8282; 0.2) |
| 4 | WT | WT | WT | Inconclusive† | WT | WT (8371) |
| 5 | WT | WT | WT | G13D | WT | Not tested |
| 6 | G12A | G12A | G12A | WT | G12A | No result† |
| 7 | G12S, G13D | G12S | G12S, no call 13† | Inconclusive† | Failed† | G12S (53/825; 0.06) |
| 8 | G12V, G13D | G12V | G12V | G12S, G12V | G12V | G12V (244/1289; 0.19), G13D (53/1289; 0.04) |
| 9 | WT | WT | WT | Not analyzed‡ | Failed† | G12V (43/106; 0.41) |
| 10 | WT | WT | 12WT, no call 13† | WT | Failed† | WT (18) |
| 11 | WT | WT | Mixed sequence§ | Not analyzed‡ | Failed† | No result† |
| 12 | G12V | G12V | G12V | G12D, G13D | G12V | G12V (2443/4707; 0.52) |
| 13 | WT | WT | WT | G13D | WT | WT (9223) |
| 14 | WT | WT | WT | Inconclusive† | WT | WT (9921) |
| 15 | G12D | G12D | G12D | G12D, G13D | G12D | G12D (4178/8006; 0.52) |
| 16 | WT | WT | WT | Inconclusive† | WT | WT (7001) |
| 17 | G13D | G13D | No call 12 or 13† | G13D | G13D | Not tested|| |
| 18 | G12A | G12A | G12A | G12A, G13D | G12A | Not tested|| |
| 19 | WT | WT | WT | G13D | WT | WT (8891) |
| 20 | G12D | G12D | G12D | G12D | G12D | G12D (2513/4304; 0.58) |
| 21 | G13D | G12D | WT | G12D, G13D | G12D, G13D | G12D (761/6260; 0.12), G13D (1639/6260; 0.26) |
| 22 | WT | WT | WT | Inconclusive† | WT | WT (2908) |
| 23 | G12D | G12D | WT | Inconclusive† | G12D | G12D (2115/7262; 0.29) |
| 24 | WT | WT | No amplification¶ | WT | Failed† | WT (71) |
| 25 | WT | WT | WT | G13D | WT | WT (2477) |
| 26 | WT | WT | WT | Inconclusive† | WT | WT (5887) |
| 27 | G12V | G12V | G12V | G12V | G12V | G12V (1812/6404; 0.28) |
| 28 | WT | WT | WT | Inconclusive† | WT | WT (8696) |
| 29 | WT | WT | G12V | G13D | WT | WT (3876) |
| 30 | G13D | G13D | G13D | G13D | G13D | G13D (4697/9754; 0.48) |
| 31 | WT | WT | No amplification | G12D, G13D | G13D | No result† |
| 32 | WT | WT | WT | G13D | WT | WT (5048) |
| 33 | G12V | G12V | WT | G12V, G13D | G12V | G12V (505/1042; 0.48) |
| 34 | WT | WT | WT | G13D | WT | WT (6329) |
| 35 | G13D | G13D | G13D | G13D | G13D | G13D (2578/7158; 0.36) |
| 36 | G12V, G13D | G12V | G12V | Inconclusive† | G12V | Not tested|| |
| 37 | G12D | G12D | G12D | G12D, G13D | G12D | G12D (14/61; 0.23) |
| 38 | G13D | WT | 12WT, no call 13† | G13D | G13D | G13D (1539/5423; 0.28) |
| 39 | WT | WT | WT | G13D | WT | Not tested|| |
| 40 | G12D | G12D | G12D | Inconclusive† | G12D | G12D (1884/4565; 0.41) |
PCR = polymerase chain reaction; WT = wild type.
*Numbers in parentheses indicate the proportion of sample DNA containing the mutant allele.
†Service was unable to conclusively determine genotype.
‡Sample was determined to be unsuitable for subsequent hybridization.
§Multiple observed sequences precluded deconvolution of sequence traces.
||Samples no longer available for testing.
¶KRAS sequence could not be amplified by PCR.
**The single number in parentheses refers to the total sequence reads for that sample when all were WT, and for MT, the fraction reflects the number of sequence reads for a given mutation over the total number of sequence reads for that sample, followed by the numerical fraction of that ratio.