Figure 6.

Spectral image maps of infrared absorbance at wavenumbers (cm⁻¹) chosen to highlight the three putative regions (stem cell, transit-amplifying cell, and terminally-differentiated cell) of human cornea. A: An unstained cryosection of corneal limbus from which the maps were taken. The red rectangle shows the precise area from which the mid-infrared (IR) spectral map was acquired. The dotted red line shows the approximate location of the basal limbal stem cells. The green line indicates the superficial epithelium. B: The limbal region immunolabeled with a green fluorescent marker (keratin 15) for corneal SCs. The cell nuclei have been stained with DAPI (4',6-diamidino-2-phenylindole; blue), and the basement membrane has been immunolabeled for laminin (red). C: Mid-IR spectral map showing absorbance for 1,040 cm⁻¹; D: mid-IR spectral map showing absorbance for 1,107 cm⁻¹; E: mid-IR spectral map showing absorbance for 1,225 cm⁻¹; F: mid-IR spectral map showing absorbance for 1,400 cm⁻¹; G: mid-IR spectral map showing absorbance for 1,525 cm⁻¹; H: mid-IR spectral map showing absorbance for 1,558 cm⁻¹; and I: mid-IR spectral map showing absorbance for 1,728 cm⁻¹.