Fig. 15.3.

Intravital images of mesenteric panels before and after growth factor overexpression (A–F). Intravital images obtained by −4 objective lens of patent vessels. Vessels on d 1 (A, C, E) and the same vessels imaged 6 d later (B,D,F). Vessels are visible due to the contrast of the blood flow. An increase in functional vessel area (FVA) is determined by an increase in patent vessel area (G), the magnitude of this increase is measured in H. ***p < 0.001 versus enhanced green fluorescent protein (eGFP), analysis of variance (ANOVA), ..p < 0.01 d 1 versus d 7, t-test. Scale bar 1 mm, n = 5 all groups, mean ± standard error of the mean (SEM). Ang-1 angiopoietin 1, VEGF vascular endothelial growth factor