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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Methods Mol Biol. 2009;467:251–270. doi: 10.1007/978-1-59745-241-0_15

Fig. 15.4.

Fig. 15.4

Growth factor expression stimulates endothelial cell (EC) proliferation. Fluorescent staining of mesenteries after injection of adenovirus-enhanced green fluorescent protein (Ad-EGFP) (control) or Ad-growth factors. Isolectin IB4-TRITC (red) stains endothelial cells ECs, Hoechst 33324 (blue) stains all mesenteric nuclei and antibodies to Ki67-AF488 (green) to detect proliferating cells. Overlaying of the stack images is used to calculate the number of proliferating endothelial cells (PECs). Images are triple stained with TRITC-streptavidin and biotinylated GSL lectin IB4 (EC, red). Alexa Fluor 488-labeled goat antimouse immunoglobulin G and mouse monoclonal anti-Ki67 antibody (proliferating cells, green) and overlay. The PECs can be distinguished from other cells by their position within the vessel wall. White arrow demonstrates a PEC. VEGF vascular endothelial growth factor. Scale bar 40 μm