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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Mol Cell. 2008 May 9;30(3):267–276. doi: 10.1016/j.molcel.2008.03.023

Figure 2. Colocalization Activates Multiple Checkpoint Readouts.

Figure 2

(A) Sml1 is degraded upon colocalization. A wild-type strain was left untreated or was treated 100 ug/ml Zeocin for 4 hr. Strains expressing checkpoint fusions −array and +array were arrested with nocodazole, and galactose was added to induce fusions. The westerns were blotted against endogenous Sml1 and Rad53-HA. A sml1 delete strain served as control for specificity (last lane).

(B–D) Strains containing fusions −array (B), +array (C), and +array, rad9 (D) were arrested in nocodazole, induced with galactose for 1 hr while arrested, and then released into media with α factor. A time course of FACS analysis is shown starting at 2 hr (120′) into galactose induction.

(E) Analysis of Rad53-HA by western blot of samples taken from (B)–(D).