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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Mol Cell. 2008 May 9;30(3):267–276. doi: 10.1016/j.molcel.2008.03.023

Figure 6. CDK Promotes Rad53 Activation.

Figure 6

(A and B) Strains were arrested in G1 with α factor and G2/M with nocodazole, and galactose was added to induce checkpoint fusions.

(C) Checkpoint fusions were induced in nocodazole-arrested isogenic wild-type and analog (1NM-PP1)-sensitive, cdc28-as, strains containing a LacO array. Both strains were treated as follows: inhibitor alone (5 uM), galactose plus inhibitor, and galactose alone.

(D) The strain carrying the cdc28-as allele, checkpoint fusions, and array was arrested with nocodazole and induced with galactose. After 3 hr, the culture was split and inhibitor was added.