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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Mol Cell. 2008 May 9;30(3):267–276. doi: 10.1016/j.molcel.2008.03.023

Figure 7. Rad9 CDK Site Mutant Is Checkpoint Deficient.

Figure 7

(A) Isogenic strains carrying wild-type Rad9-Flag or Rad9-18A-Flag were α factor arrested, nodocazole arrested, allowed to cycle, or treated with cdc28-as inhibitor 1NM-PP1.

(B) Ddc1-GFP-LacI and Ddc2-GFP-LacI checkpoint fusions were induced in nocodazole-arrested Rad9-Flag and Rad9-18A-Flag strains. Zeocin was added to Rad9-Flag, Rad9-18A-Flag, and rad9Δ strains.

(C) Rad9-Flag, Rad9-18A-Flag, and rad9Δ cycling strains were left untreated or treated with Zeocin at 100 ug/ml, 200 ug/ml, and 2 mg/ml final concentration for 2 hr.