Table 2. Substrate-binding mutants of tartrate dehydrogenase.
| Enzyme form | kcat (min−1) | kcat (%) | Kmalate (µM) | kcat/Kmalate (M−1 s−1) | KNAD (µM) | kcat/KNAD (M−1 s−1) |
|---|---|---|---|---|---|---|
| Native | 390 ± 4 | 100 | 79 ± 3 | 8.2 × 104 | 25.0 ± 0.4 | 2.6 × 105 |
| R98L | —† | |||||
| R98Q | 10.5 ± 0.1 | 2.6 | 98 ± 2 | 1.8 × 103 | 96 ± 2 | 1.8 × 103 |
| R108L | 5.2 ± 0.9 | 1.3 | 180 ± 30 | 4.8 × 102 | 240 ± 40 | 3.6 × 102 |
| R108Q | 34.6 ± 1.8 | 8.7 | 93 ± 18 | 6.2 × 103 | 135 ± 26 | 4.3 × 103 |
Not expressed in stable form.