Table 3. Metal-binding mutants of tartrate dehydrogenase.
| Enzyme form | kcat (min−1) | kcat (%) | KMn (mM) |
|---|---|---|---|
| Native | 390 ± 4 | 100 | 0.05 ± 0.01 |
| D225A | 0.91 ± 0.15 | 0.2 | 1.0 ± 0.4 |
| D250A | 5.3 ± 0.8 | 1.4 | 0.6 ± 0.1 |
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