. 2010 May 15;66(Pt 6):741–744. doi: 10.1107/S0907444910012436

Table 2. Summary scaling statistics for three data sets, comparing the conventional approach of background rejection with the new approach of ice-ring subtraction.

The first two data sets were collected from C222 crystals of human acidic fibroblast growth factor mutants (Lee et al., 2008 ▶). ‘FGF-d’ was used for the structure determination of mutant L26N/D28A (PDB code 3ba7). ‘FGF-e’ was an ice-affected data set for mutant K112N/N114A that was replaced prior to structure determination (PDB code 3bag). ‘Sxa63’ is a data set from a P222 crystal of the Pyrococcus furiosus box C/D ribonucleoprotein particle that was also replaced prior to structure determination (Li et al., submitted work). Resolution shells affected by ice rings where the new procedure improves the completeness are shown in bold.

FGF-d					FGF-e					Sxa63
	Background rejection		Ice-ring subtraction			Background rejection		Ice-ring subtraction			Background rejection		Ice-ring subtraction
Resolution (Å)	R_merge	Completeness (%)	R_merge	Completeness (%)	Resolution (Å)	R_merge	Completeness (%)	R_merge	Completeness (%)	Resolution (Å)	R_merge	Completeness (%)	R_merge	Completeness (%)
∞–4.00	0.049	94	0.050	95	∞–5.12	0.048	100	0.047	100	∞–6.79	0.070	100	0.076	100
4.00–3.18	0.075	70	0.071	99	5.12–4.08	0.062	100	0.062	100	6.79–5.42	0.088	100	0.088	100
3.18–2.78	0.083	96	0.083	97	4.08–3.57	0.117	37	0.147	98	5.42–4.75	0.097	100	0.099	100
2.78–2.52	0.091	87	0.091	87	3.57–3.24	0.130	65	0.125	100	4.75–4.32	0.102	100	0.102	100
2.52–2.34	0.096	76	0.097	76	3.24–3.01	0.143	99	0.120	98	4.32–4.01	0.111	100	0.116	100
2.34–2.20	0.128	51	0.142	76	3.01–2.83	0.171	95	0.135	95	4.01–3.78	0.170	72	0.165	100
2.20–2.09	0.120	74	0.113	76	2.83–2.69	0.201	92	0.153	92	3.78–3.59	0.404	47	0.346	100
2.09–2.00	0.159	77	0.159	77	2.69–2.57	0.277	92	0.214	91	3.59–3.43	0.233	100	0.229	100
2.00–1.93	0.190	77	0.174	78	2.57–2.48	0.274	91	0.205	90	3.43–3.30	0.243	100	0.258	100
1.93–1.86	0.204	69	0.226	78	2.48–2.39	0.325	89	0.233	89	3.30–3.19	0.273	100	0.327	100
1.86–1.80	0.244	77	0.236	77	2.39–2.32	0.418	89	0.244	88	3.19–3.09	0.339	100	0.398	100
1.80–1.75	0.280	77	0.283	77	2.32–2.25	0.595	33	0.645	87	3.09–3.00	0.387	100	0.439	99
∞–1.75	0.081	77	0.081	83	∞–2.25	0.124	82	0.122	94	∞–3.00	0.119	93	0.125	100