Table 1. Oligonucleotides used in this study.

Primer	Sequence (5′ to 3′)	Purpose of Use
CihC F	GGA GGA AAA TGG ATC GAT GAA GAG ACA ATG	amplification of cihC
CihC R	ATT TAA GCT ATC TGC CAT TC	amplification of cihC
CihC Bam	TAT TGG ATC CGA TTT ATT ATT TGA CGA AG	generation of expression plasmid
CihC Hinc	ATT TAA GCT ATC TGC CAT TC	generation of expression plasmid
CihCΔ83F	GGA CAA CAG GGA TCC ATA G	construction of deletion mutant
CihCΔ122F	GAG ATT AGT AAG GGA TCC AAA GAG	construction of deletion mutant
CihCΔ160F	GAA GGA TCA GGA TCC GGT GGA	construction of deletion mutant
CihCΔ149R	GTG TCG ACA GTT ATG TTG TAC CG	construction of deletion mutant
CihCΔ190R	ATA TTC ATA GTC GAC TCA ATC TTC	construction of deletion mutant
CihCΔ260R	CAA AAG TCG ACT AAA GTT CTT GTG CTA GC	construction of deletion mutant
CihCΔ294R	CTT GGT CGA CTA AAT AGC CCT GTA AAG	construction of deletion mutant
CihC Prom	AAA AGG ATC CAC AAT TAC TTA TAC	construction of pCihC
CihC SphI	GTA AAT TTG CAT GCT TGC TTA AGA G	construction of pCihC