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(A). Immunoblot analysis of extracts from Giardia trophozoites and cysts. 30 µg of each extracts were separated on 12% SDS-PAGE transferred to a nitrocellulose membranes and probed with anti-Rab11 policlonal antibody. The antibody detects a single band with a molecular weight near 24 kDa. Lanes 1 and 2 correspond to trophozoites and cysts, respectively. (B). Two-dimensional electrophoresis (only one spot, pI of 8.7, was identified).
