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. Author manuscript; available in PMC: 2011 May 26.
Published in final edited form as: Vaccine. 2010 May 26;28S2:B18–B23. doi: 10.1016/j.vaccine.2010.02.002

Figure 2. Flow cytometric identification of HIV-specific B cells.

Figure 2

After paramagnetic bead enrichment of B cells from PBMCs, cells from an HIV+ subject (top flow panel) or an HIV- subject (bottom flow panel) were labeled separately with HIV-VLPs and appropriate cell markers. Detection of the anti-CD19 and HIV-VLP reagents are shown after prior gating (not shown) to exclude dead cells, CD3+ T cells, and CD14+ monocytes.