Table 4.
Ratio of radiation sensitivity of parental or wild type cells over that of radiosensitive mutants or cells with reduced Ku70/80 activity. Radiation dose to reduce surviving fraction to 0.1 [D(0.1)] was obtained by authors for each cell type from published figures. Ratio is the ratio of D(0.1) of parental or mutant cells to D(0.1) of cells with reduced activity.
| Cell Types | Ratio | Reference |
|---|---|---|
| Radiosensitive Mutant-1 | Wild/Mutant | |
| DSB Repair-deficient V79 (sxi-1 – 4) | 2.2 – 4.2 | Lee SE, 1995 (17) |
| DSB Repair-deficient CHO (xrs-6) | 7.6 | Ross GM, 1995 (21) |
| Ku80-deficient CHO (xrs-6) | 8.6 | Singleton BK, 1997 (23) |
| Ku80-deficient ES | 3.7 | Nussenzweig A, 1997 (19) |
| Ku80-deficient CHO (xrs-6) | 5.0 | Marangoni, E 2000 (18) |
| Ku80-deficient CHO (xrs-6) | 6.6 | Okayasu R, 2006 (20) |
| Ku80-deficient MEF | 3.9 | Wang H, 2008 (24) |
| Ku80-deficient MEF | 5.9 | Schulte-Uentrop L, 2008(22) |
| Radiosensitive Mutant-2 | Transfectant/Mutant | |
| NHEJ-deficient V79 (XR-V15B) | 2.3 (Ku86*) | Smider V, 1994 (26) |
| NHEJ-deficient V79 (XR-V15B) | 1.0 (Ku70*) | Smider V, 1994 (26) |
| NHEJ-deficient V79 (XR-V15B) | 3.0 (Ku70 & 86*) | Smider V, 1994 (26) |
| Ku80-deficient CHO (xrs-6) | 3.4 (Ku80*) | Taccioli GE, 1994 (27) |
| DSB Repair-deficient CHO (xrs-6) | 6.6 (Ku80*) | Ross GM, 1995 (21) |
| NHEJ-deficient V79 (XR-V15B) | 1.6 - >3.0 (Ku86*) | Errami A, 1996 (25) |
| Knock out | Parent/Knock out | |
| Ku70−/− ES | 2.3, 3.2 | Gu Y, 1997 (29) |
| Ku70−/− (Ku70−/−/RAD54−/−) DT40 | 0.8 (6.0) | Utsumi H, 2001 (30) |
| Ku70−/− DT40 | 1.5 | Adachi N, 2001 (28) |
| Ku70−/− MEF | 5.2 | He F, 2003 (9) |
| Ku antisence | Parent/Antisense | |
| MRC5V1 Human fibroblasts | 1.8 (Ku86*) | Marangoni E, 2000 (32) |
| M059K Malignant glioma | 1.8 (Ku86*) | Belenkov AI, 2002 (31) |
| RERFV1 Human lung sq.c.ca | 1.5 – 1.7 (Ku70*) | Omori S, 2002 (33) |
| FSa-II Mouse fibrosarcoma | 1.5 (Ku70-virus*) | Li GC, 2003 (10) |
| siRNA | Parent/Transfectant | |
| HeLa Human cervical ca. | 1.4 (Ku70*) | Ayen, IS, 2005 (34) |
| A549 Human Lung ca. | 1.5∞ (Ku80*) | Nimura Y, 2007 (35) |
| H1299 Human Lung ca. | 1.4∞ (Ku80*) | Nimura Y, 2007 (35) |
| RT112 Human Bladder ca. | 1.2 (Ku80*) | Negroni A, 2008 (36) |
| DNKu70/80 | Parent/Transfectant | |
| CHO cells | 2.0 (DNKu80*) | Marangoni, E, 2000 (18) |
| Rat-1 fibroblasts | 1.5 – 1.9 (DNKu70*) | He, F, 2007 (9) |
| U-87 Human Glioblastoma | 1.8 (DNKu70*) | He, F, 2007(9) |
| Rat-1 fibroblasts | 1.3 – 1.8 (DNKu70*) | Urano, M, 2008 (11) |
| DNKu70-virus | Parent/Virus-infected | |
| U-87 Human Glioblastoma | 1.8 (50 MOI**) | He, F, 2007 (9) |
| HCT8 Human Colorectal ca. | 1.5 (50 MOI**) | He, F, 2007 (9) |
| HCT8 Human Colorectal ca. | 1.3 (25 MOI**) | Present |
| HT29 Human Colorectal ca. | 1.5 (25 MOI**) | Present |
[Note]
Abbreviations: V79 & CHO=Chinese hamster lung & ovary cells; MEF=mouse embryo fibroblasts; ES=embryonic stem cells; DT40=Chicken B-lymphocyte;
*
Transfected cDNA, siRNA or fragment.
**
Virus dose.
∞
Values obtained by extrapolating to SF of 0.1.