Fig. 6.
Cleavage of the caspase substrates poly(adenosine 5′-diphosphate ribose) polymerase (PARP) and PKCδ is induced by etoposide and brefeldin A (BFA) in primary rat parotid acinar cells. Subconfluent primary cultures of parotid acinar cells were left untreated (lane 1) and treated for the indicated times with 150 μM etoposide (lanes 2–5) or 2 μM BFA (lanes 6–9). Cell lysates were immunoblotted with anti-PARP (panel A) or anti-PKCδ (panel B). The positions of the full-length and cleavage fragments are indicated on the right side of each blot. The length of treatment (in h) with etoposide and BFA is indicated on the top of each lane. Blots are representative of two independent experiments.