Fig. 7.
Cleavage of the caspase substrates PARP and PKCδ is induced by etoposide and brefeldin A (BFA) in primary rat submandibular acinar cells. Subconfluent primary cultures of submandibular acinar cells were left untreated (lane 1) and treated for the indicated times with 150 μM etoposide (lanes 2–5) or 2 μM BFA (lanes 6–9). Cell lysates were immunoblotted with anti-PARP (panel A) or anti-PKCδ (panel B). The positions of the full-length and cleavage fragments are indicated on the right side of each blot. The length of the treatment (in h) with etoposide and BFA is indicated on the top of each lane. Blots are representative of two independent experiments.