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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Exp Eye Res. 2010 Mar 31;91(1):15–25. doi: 10.1016/j.exer.2010.03.016

Figure 2. Quercetin treatment could inhibit the apoptotic pathway in ARPE-19 cells activated by H2O2.

Figure 2

RPE cells were treated with l.0 mM H2O2 with/without 50μM quercetin for 2 hrs and were then incubated with normal culture medium for 4hrs. Cells were then harvested, and total RNA was isolated and converted to cDNA. RQ-PCR analysis was then performed to determine the levels of targeted gene transcripts. (A) BAX; (B) BCL-2; (C) ratio of BCL-2/BAX; (D) FADD; (E) CASPASE-3 and (F) CASPASE-9. Bars represent the mean ± S.E. of three independent experiments and indicate fold change relative to control cells. (*: p < 0.05)