Figure 5. Memory T-cell responses to CMV-pp65 after infection of human PBMC with rMVA-pp65-IGR3.
CD8+ pp65-specific IFN-γ+ responses were generated from human PBMCs of 2 CMV-positive healthy subjects (HS1, HS2). The approach used in-vitro stimulation with rMVA-pp65-IGR3-infected autologous PBMC, after conditioning using a cocktail of CpG ODN. Details of the approaches can be found in Materials and Methods.