Table 1.

Primers used in generation of IGR3 transfer vector and PCR analysis of virus stability during passage

Target Gene	Primer Sequences
MVA IGR3 FL13	Forward: 5′ CCGGAATTCCACCTTCTATAGATCTGAG 3′
	Reverse: 5′ GGTACCGCGCGTGTCGACTCAAATGAGATAAAGTGAAAATATATATC 3′
MVA IGR3 FL23	Forward: 5′ CATTTGAGTCGACACGCGCGGTACCGAATAAAAATGTTTTTGTTTAACCACTGCA 3′
	Reverse: 5′ CGCGAATTCGGAATTGGGAACCTCTAAAAG 3′
MVA IGR3 (qPCR)	Forward: 5′ AACACCCGCACAAGTCTG 3′
	Reverse: 5′ TCCGCTCCCATTCAATTC 3′
CMV pp65 (qPCR)	Forward: 5′ A TCAAACCGGGCAAGATCTCGC 3′
	Reverse: 5′ ATCGTACTGACG CAGTTCCACG 3′