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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Virology. 2010 May 6;403(2):103–110. doi: 10.1016/j.virol.2010.04.011

Figure 2.

Figure 2

PCR amplification of the recombinant env gene of FeLV-B from the genomic DNA of naturally and experimentally infected cats. Genomic DNA samples were amplified by PCR using forward and reverse primers specific for the endogenous FeLV-related CFE-6 element and exogenous FeLV-945, respectively. A. Genomic DNA was examined from naturally occurring thymic lymphomas (1037 thy, 1110 thy and 1100 thy) and multicentric lymphomas (1345 liver, 1049 spleen, and 945 kidney). B. Genomic DNA was examined from experimentally induced thymic lymphomas (O26 thy, O24 thy, and 981-2 thy) and multicentric lymphomas (N90 liver, N91 liver, and N92 liver). Indicated by the arrow is the predicted amplification product of ~1.1-kb. Genomic DNA from uninfected FEA cells, and from canine D-17 cells infected with FeLV-B/Gardner-Arnstein were examined as negative and positive controls, respectively.