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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Virology. 2010 May 6;403(2):103–110. doi: 10.1016/j.virol.2010.04.011

Table 2.

Detection of FeLV-B proviral DNA in diseased tissues from experimentally infected cats.a

Animal Diseaseb Inoculumc Reference S. Blot PCR
O3 Thymic lymphoma FeLV-A/61E Chandhasin et al., 2005 + +
O4 Thymic lymphoma FeLV-A/61E Chandhasin et al., 2005 + +
O5 Thymic lymphoma FeLV-A/61E Chandhasin et al., 2005 + +
O6 Thymic lymphoma FeLV-A/61E Chandhasin et al., 2005 + +
O23 Thymic lymphoma 61E945L Chandhasin et al., 2005 + +
O24 Thymic lymphoma 61E945L Chandhasin et al., 2005 + +
O25 Thymic lymphoma 61E945L Chandhasin et al., 2005 + +
O26 Thymic lymphoma 61E945L Chandhasin et al., 2005 + +
981-2 Thymic lymphoma LC-FeLV (FeLV-A+B) Levy, Fish & Baskin, 1988 − −
981-3 Thymic lymphoma LC-FeLV (FeLV-A+B) Levy, Fish & Baskin, 1988 + +
J5-1 Thymic lymphoma LC-FeLV (FeLV-A+B) Levy, Fish & Baskin, 1988 − −
N65 Thymic lymphoma 61E/945SL Chandhasin et al., 2005 − +
N67 Thymic lymphoma 61E/945SL Chandhasin et al., 2005 − +
N89 Unknown 61E/945SL Chandhasin et al., 2005 − ±
N90 Multicentric lymphoma 61E/945SL Chandhasin et al., 2005 − ±
N91 Multicentric lymphoma 61E/945SL Chandhasin et al., 2005 − ±
N92 Multicentric lymphoma 61E/945SL Chandhasin et al., 2005 − ±
586-4 Feline infectious peritonitis FeLV-A+B Levy, Fish & Baskin, 1988 + +
585-2 Transient lymphadenopathy LC-FeLV (FeLV-A+B) Levy, Fish & Baskin, 1988 − +
586-3 Healthy LC-FeLV (FeLV-A+B) Levy, Fish & Baskin, 1988 + +
586-5 Healthy Mock Levy, Fish & Baskin, 1988 − −
585-1 Healthy Mock Levy, Fish & Baskin, 1988 − −
a

Presence (+) or absence (−) of FeLV-B proviral DNA as detected by Southern blot analysis or polymerase chain reaction (PCR) amplification. ± = weakly detectable.

b

Tumor tissues were analyzed from animals with lymphoma. Lymph node was examined from animals 586-3 and 586-4. PBMC were examined from animals 585-1, 585-2 and 586-5. Lung, lymph node and liver was examined from N89.

c

Animals were inoculated experimentally with FeLV-A/61E, a synthetic recombinant of FeLV-A/61E containing either the LTR of FeLV-945 (61E945L) or the SU gene and LTR of FeLV-945 (61E/945SL), a natural myc-containing isolate of FeLV (LC-FeLV) in the presence of helper viruses FeLV-A and FeLV-B (FeLV A+B), or the helper viruses alone (FeLV-A+B). All animals were inoculated intraperitoneally except N65 and N67, which were inoculated intradermally.