Skip to main content
. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Microvasc Res. 2010 Jan 4;80(1):75–88. doi: 10.1016/j.mvr.2009.12.010

Figure 10. Deletion of the actin- and cortactin-binding domains of nmMLCK2 ablates colocalization with cortactin in S1P-stimulated live EC.

Figure 10

HPAEC were cotransfected with EGFP-nmMLCK2Nterm and cortactin-DsRed constructs and then subjected to live cell imaging as described in Methods. The panels represent images from an extended movie (see Supplemental Movie 6) depicting dynamic colocalization of cortactin-DsRed and EGFP-nmMLCK2Nterm in a single EC under basal conditions (A), after thrombin stimulation (B), and subsequent S1P stimulation (C–E). Yellow indicates areas of colocalization. ICQ quantitation for nmMLCK2Nterm-cortactin colocalization in outlined lamellipodia is shown for each panel.