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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Microvasc Res. 2010 Jan 4;80(1):75–88. doi: 10.1016/j.mvr.2009.12.010

Figure 5. Dynamic colocalization of actin-GFP and cortactin-DsRed with intensity correlation analysis applied to live-cell imaging.

Figure 5

HPAEC were cotransfected with actin-GFP and cortactin-DsRed constructs and then subjected to live cell imaging as described in Methods. The panels represent images from an extended movie (see Supplemental Movie 1) depicting dynamic colocalization of cortactin-DsRed and actin-GFP in a single EC under basal conditions (A), after thrombin stimulation (B), and subsequent S1P stimulation (C–F). Yellow indicates areas of colocalization. ICQ quantitation for actin-cortactin colocalization in outlined lamellipodia is shown for each panel.