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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Microvasc Res. 2010 Jan 4;80(1):75–88. doi: 10.1016/j.mvr.2009.12.010

Figure 7. Reduced colocalization of cortactinΔSH3-DsRed with EGFP-nmMLCK1 in live human lung EC.

Figure 7

HPAEC were cotransfected with EGFP-nmMLCK1 and cortactinΔSH3-DsRed constructs and then subjected to live cell imaging as described in Methods. The panels represent images from an extended movie (see Supplemental Movie 3) depicting dynamic colocalization of cortactinΔSH3-DsRed and EGFP-nmMLCK1 in a single EC under basal conditions (A), after thrombin stimulation (B), and subsequent S1P stimulation (C–E). Yellow indicates areas of colocalization. ICQ quantitation for nmMLCK1-cortactinΔSH3 colocalization in outlined lamellipodia is shown for each panel.