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. 2010 Jan 31;38(10):3414–3422. doi: 10.1093/nar/gkq001

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Illustration of the proposed bending measurement method. A hypothetical nucleic acid structure is considered, involving 10 bases. First, a coordinate frame is attached to each base, using a standard procedure (black frames). Three groups of bases are then chosen: the initial (bases 1–3 and 10, red), middle (bases 4 and 9, green) and final (bases 6 and 7, blue) group. Bases 5 and 8 do not belong to any group. For each group, the average frame is computed. The global bending magnitude is defined as the angle between the average frame vector i₃ of the initial group and the average frame vector f₃ of the final group. The bending direction is evaluated with respect to the average frame of the middle group. A detailed description of the method is given in Section S1 of the Supplementary Data.