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. 2010 Feb 5;38(10):3209–3221. doi: 10.1093/nar/gkq026

Figure 1.

Figure 1.

Recombinant MSL derivatives investigated in this study. (A) Schematic representation of MSL2 domain organization and various MSL2 expression constructs. All MSL2 constructs contain a C-terminal FLAG tag. Numbers correspond to the amino acid positions in full-length MSL2. (B) Alignment of orthologue CXC domains from the Drosophila melanogaster MSL2 protein (DmCXC) and from the H. sapiens protein KIAA1585 (HsCXC). Black boxes highlight the conserved cysteines (37). Arrows indicate the introduced point mutations. (C) Coomassie-stained SDS–polyacrylamide gel of purified recombinant MSL proteins.