Figure 2.

Binding of recombinant MSL proteins to a DNA high-affinity site in vitro. Electrophoretic mobility shift assay. Increasing concentrations (from 5 to 250 nM) of (A) MSL2 and MSL1 or (B) MSL2 and the MSL2–MSL1 complex were incubated with radiolabeled 40 bp DBF12-L15 dsDNA and protein–DNA complexes were separated from unbound DNA in non-denaturing agarose gels. (C) Binding curves obtained from quantification of (A) and (B) and fitting to a standard bimolecular model (see ‘Materials and Methods’ section).