Figure 4.

Competition assays to determine the binding preference of MSL2 for different nucleic acids. (A) Electrophoretic mobility shift assay. Fifty nanomolar of MSL2 was incubated with the radiolabeled 40 bp DBF12-L15 dsDNA and then increasing concentrations (from 5 to 2080 nM) of unlabeled competitor nucleic acids were added. Representative examples of competition with ds- and ssDNA are shown. (B) Competition curves obtained from quantification of data such as in (A), but also including ssRNA and dsRNA as competitors and fitting to the model as described in ‘Materials and Methods’ section.