Table 1.

Comparison of binding affinities of different recombinant MSL2 derivatives to 40 bp DBF12-L15 dsDNA and RNA

MSL protein	KD dsDNA [nM]	KD dsRNA [nM]
MSL2	33 ± 13	21 ± 4
ΔCXC	189 ± 18	29 ± 12
ΔRING	51 ± 5	23 ± 5
ΔPro / Bas	61 ± 5	37 ± 10
C544A/C546A	82 ± 4
Y547A	81 ± 5
HsCXC	33 ± 9
MSL2-MSL1	26 ± 15
MSL1	no binding

K_D values were calculated from binding curves obtained from quantification of EMSA gels and fitting to a standard bimolecular model. Mean values are shown plus and minus the standard deviation from several replicates obtained with at least two independent protein preparations. For details see the ‘Materials and Methods’ section.