Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Feb 15;38(10):3489–3501. doi: 10.1093/nar/gkq027

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — The strategy and design of a bacterial assay for detecting RNA loop–loop interactions that inhibit U1A–hpII-mediated antitermination complex formation. (A) A bacterial two-plasmid system based on phage λ N-mediated antitermination for detection of RNA–polypeptide interactions. (B) The secondary structure of the region of the RNA transcript containing the target U1 hpII and downstream RNA stem–loop library. The randomized nucleotides are indicated by N, and the regions corresponding to the PstI, BsrGI, and BamHI sites are shown in italic. (C) Possible mechanism for the disruption of antitermination complex formation by antisense RNA stem–loops.